Enyimba suffered a disappointing 1-0 home defeat to Ikorodu City in their Matchday 13 encounter at the Enyimba International Stadium, Aba, on Sunday.
Tomisin Adebayo scored the decisive goal 20 minutes from time to hand Ikorodu City all three points.
Defending champions Rangers were held to a 1-1 draw by Niger Tornadoes at the Bako Kontagora Stadium in Minna.
John Zakka put the hosts ahead in the 25th minute before Waheed Adebayo equalised for Rangers 15 minutes from time to ensure both sides shared the spoils.
In Ilorin, Kwara United and Rivers United also played out a 1-1 draw in an entertaining encounter.
Emmanuel Ekpeyong headed Kwara United into the lead in the 71st minute, but the hosts’ celebrations were short-lived as Wasiu Falolu restored parity for Rivers United just a minute later.
Katsina United claimed a 2-1 victory over Nasarawa United at the Muhammadu Dikko Stadium.
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Luis Dadong and Mubarak Sani scored in the first half to give the hosts a comfortable advantage before Sunday Aluku pulled one back for Nasarawa United in stoppage time.
Warri Wolves also recorded a 2-1 victory over Sporting Lagos.
Sabastine Uche opened the scoring for Warri Wolves six minutes before the half-time break, while Chisom Orji doubled their advantage in the 55th minute.
Abdullahi Abdulraqeeb reduced the deficit for Sporting Lagos shortly after the hour mark, but Warri Wolves held on to secure maximum points.
Meanwhile, Inter Lagos celebrated their first-ever home victory in the Nigeria Premier Football League (NPFL) with a 2-1 win over Abia Warriors.
Uchenna Njoku and John Danga scored in the first half to put Inter Lagos in control before Ikoi Ettah pulled one back for Abia Warriors in the 53rd minute.
Inter Lagos, however, held on to their advantage to secure a historic home win.
Full Results
Kwara Utd 1-1 Rivers Utd
Tornadoes 1-1 Rangers
Inter Lagos 2-1 Abia Warriors
Enyimba 0-1 Ikorodu City
Katsina Utd 2-1 Nasarawa Utd
Wolves 2-1 Sporting Lagos
By Adeboye Amosu
