Rangers suffered a setback in their title bid following a 1-0 defeat against Shooting Stars in Ibadan on Wednesday.
Christian Pyagbara scored the decisive goal for Shooting Stars on 13 minutes.
Rangers remain on top of the log with 64 points from 36 games despite the defeat.
Shooting Stars boosted their chances of securing a continental ticket following the win.
Enyimba suffered a hitch in their title bid after they were held to a 1-1 draw by Bayelsa United in Aba.
Alade Balogun gave Enyimba the lead on 20 minutes, while Ekeson Okorie equalised for the visitors 10 minutes before the break.
Remo Stars moved to second position on the table after a comprehensive 4-1 victory over Heartland in Ikenne.
Stanley Joseph bagged a brace, while Franck Mawuena and Jide Fatokun scored the other goals for the hosts.
Heartland were relegated to the Nigeria National League following the defeat.
In Uyo, Akwa United boosted their survival hopes courtesy of a 3-1 win against Katsina United.
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A brace from Sani Suleiman and Osoba Kabir helped the Promise Keepers claimed maximum points in the game.
Bello Lukman scored Akwa United’s consolation goal.
In another matchday 35 tie, Kwara United moved up the log courtesy of a 2-0 win against Kano Pillars.
Dehinde Ibrahim gave Kwara United the lead in the 28th minute, while Isaiah Ejeh added the second from the penalty spot on the hour mark.
At the Mobolaji Johnson Arena, Onikan, Sporting Lagos fell to a 2-1 defeat against Plateau United.
Izuchukwu Chimezie and Bernard Mundi scored twice for the visitors in the first half.
The hosts reduced the deficit through Jonathan Alukwu on 69 minutes.
Elsewhere, Chisom Okereke and Divine Nwachukwu were on target as Bendel Insurance pip Rivers United 2-1.
Shedrack Asiegbu scored Rivers United’s only goal of the game.
FULL RESULTS
Gombe Utd 1-3 Abia Warriors
Bendel Insurance 2-1 Rivers Utd
Enyimba 1-1 Bayelsa Utd
Akwa Utd 3-1 Katsina Utd
Remo Stars 4-1 Heartland
Tornadoes 3-2 Lobi Stars
Kwara Utd 2-0 Kano Pillars
Sporting Lagos 1-2 Plateau Utd
3SC 1-0 Rangers
